Coding

Part:BBa_K5048066:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-23)


LAP-GST


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1429
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2472
    Illegal BglII site found at 2570
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1429
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1429
    Illegal NgoMIV site found at 678
    Illegal NgoMIV site found at 1944
    Illegal AgeI site found at 310
    Illegal AgeI site found at 1966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2292


Design Notes

GST: Glutathione S-transferase from Schistosoma japonicum. We implement the GST tag for better specificity for protein detection. We also try to introduce LAP-GST fragment into plasmid backbone pET-28a and pBAD(Fig 1)(Fig 2).

Fig 1 Map for plasmid LAP-GST

Fig 2 Map for plasmid pBAD-LAP-GST


Source

We used amplified segments from vector pBAD and plasmid LAP-His(BBa_K5048058).


References